Fig. 2. Myriocin induces transcriptional factors activation. CF bronchoepithelial cells were treated or not with Myr (5 hours for TFEB evaluation and 12 hours for PPARγ; C, FOXO1A). Quantification of the transcriptional factor nuclear translocation by western blot on nuclear extracts and normalized on Lamin A/C: A, TFEB; B, PPARγ; C, FOXO1A. Protein signals were quantified by densitometry analysis and the normalized values reported in the graphs; two‐tailed unpaired Student's T‐test.